Journal of the Brazilian Chemical Society | |
LC-ESI-MS determination of quassinoids isobrucein B and neosergeolide in Picrolemma sprucei stem infusions | |
Adrian M. Pohlit2  Valquíria A. P. Jabor1  Rodrigo C. Das N. Amorim2  Ellen C. Costa E Silva2  Norberto P. Lopes1  | |
[1] ,Instituto Nacional de Pesquisas da Amazônia Coordenação de Pesquisas em Produtos Naturais Manaus AM ,Brazil | |
关键词: caferana; cafe lane; Picrolemma sprucei; Picrolemma pseudocoffea; neosergeolide; isobrucein B; LC-MS/MS; | |
DOI : 10.1590/S0103-50532009000600010 | |
来源: SciELO | |
【 摘 要 】
Infusions of the stems of Picrolemma sprucei (pseudonym: P. pseudocoffea) are used in the Amazon regions of Peru, Brazil and French Guiana as antimalarials among other uses. They contain the bitter quassinoids isobrucein B (1) and neosergeolide (2) that have important antimalarial and toxic properties among others. In this study, an LC-(+)-ESI-MS/MS method was developed and applied to the determination of 1 and 2 in a common remedy prepared by infusing 1 g of dry, powdered stems of P. sprucei in 1 L of boiling water. Isolated 1 and 2 were used in calibration ranges of 0.25 to 5 µg mL-1 and 0.5 to 10 µg mL-1, respectively, with the internal standard phloroglucinol at 4.0 µg mL-1. Good linearity, precision and accuracy were observed for both compounds. The concentrations of 1 and 2 in the stem infusions were found to be 60.1 and 774 µg L-1, respectively.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
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