Hepatotoxin microcystin-LR extraction optimization

Journal of the Brazilian Chemical Society

Maria Estela Silva-stenico²  Romeu Cantúsio Neto¹  Ivânio R. Alves¹  Luiz Alberto B. Moraes¹  Tânia K. Shishido²  Marli Fátima Fiore² 

[1] ,Universidade de São Paulo Centro de Energia Nuclear na Agricultura Piracicaba SP ,Brazil

关键词: LC-MS;    Q-TOF;    Microcystis aeruginosa;    microcystin;   

DOI  :  10.1590/S0103-50532009000300019

来源: SciELO

【 摘 要 】

Several cyanobacterial genera produce toxic secondary metabolites, the most well-known of which are the hepatotoxic microcystins (MCYSTs). Microcystin analyses in drinking water are a requirement of the Health Ministry (Regulation 518/2004) in Brazil, but this regulation does not establish which extraction and analytical method should be used; toxin quantification is usually carried out by ELISA (enzyme-linked immunosorbent assay) or HPLC (high performance liquid chromatography), the efficiency of which depends on the extraction method used. In this work a simple, fast and cheap method of extraction was developed for the isolation and identification of MCYSTs. For this, the strain Microcystis aeruginosa NPLJ-4, reported to be a MCYST-LR producer, was selected. Eight different treatments were tested to determine the best MCYST extraction. Samples were applied in LC-MS (liquid chromatography-mass spectrometry), ELISA and Q-TOF (quadrupole time-of-flight). The most efficient extraction was achieved by sonicating samples diluted in water. The proposed method permits rapid sample processing, and establishes an extraction method for both the analysis and identification of MCYST-LR and other variants.

【 授权许可】

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