Research on fluorescence detection method of Microcystis aeruginosa

2nd International Conference on Measurement Instrumentation and Electronics

物理学;无线电电子学

Wang, Xiao-Xiong^1

School of Civil and Environmental Engineering, Shenzhen Polytechnic, Shenzhen

518055, China^1

关键词: Aeruginosa;    Cell density;    Dead cells;    Fluorescence detection;    Fluorescence staining;    Microcystis aeruginosa;    Pi staining;    Red fluorescent;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/870/1/012026/pdf
DOI  :  10.1088/1742-6596/870/1/012026

来源: IOP

【 摘 要 】

The paper studied the viability determination of Microcystis aeruginosa by FDA and PI staining. The staining results were measured by fluorescence microscopy. The results indicated that viable and dead cells were stained as bright green and red fluorescent respectively by FDA and PI. Through PI-FDA dual color fluorescence staining, the color of green and red distinct obviously by fluorescence microscope. The staining rate has relation with the cell density. If the cell density of M. aeruginosa was 1.0×107-1.0×109cell•mL-1, the staining rate would be 100.0% or 98.0% by PI and of FDA respectively.