Fluorescence detection of Trace Dimethylamine and Diethylamine in Natural Water by HPLC with O-phthalaldehyde and 7-chlore-4nitrobenzo-2-oax-1, 3-diazole

2018 International Conference on Advanced Technologies in Energy, Environmental and Electrical Engineering

能源学;生态环境科学;无线电电子学

Gao, P.F.^1 ; Zheng, J.S.^1 ; Zhang, S.M.^1 ; Wen, X.J.^1

College of Environmental Science and Engineering, Xiamen University of Technology, Xiamen

361024, China^1

关键词: Aliphatic primary amines;    Correlation coefficient;    Derivatization reagent;    Detection limits;    Fluorescence detection;    Natural water samples;    Precolumn derivatizations;    Selective detection;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/223/1/012056/pdf
DOI  :  10.1088/1755-1315/223/1/012056

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

A method of detecting dimethylamine (DMA) and diethylamine (DEA) was developed by HPLC with pre-column derivatization and fluorescence detection in this paper. Two fluorescent derivatization reagents were chosed to used in the method including o-phthalaldehyde (OPA) and 7-chlore-4-nitrobenzo-2-oax-1,3-diazole (NBD-Cl). Aliphatic amines in the natural water were labeled in two steps, the water samples were firstly reacted with OPA to eliminate aliphatic primary amines interferences, and then NBD-Cl could be used to selective detection of secondary amines, including dimethylamine and diethylamine. The detection limits of the method were 0.1 nmol/L and 10 nmol/L for DMA and DEA. Both of the linear ranges were 0.0005-0.1mol/mL with correlation coefficients above 0.9978 and 0.9988. This method is repeatable, sensitive and accurate for the detection of DMA and DEA in natural water samples.