Validation of a method using solid phase extraction and liquid chromatography for the determination of pesticide residues in groundwaters

Journal of the Brazilian Chemical Society

Sergiane S. Caldas¹  Adriana Demoliner¹  Ednei G. Primel¹ 

[1] ,Universidade Federal do Rio Grande Programa de Pós-Graduação em Química Tecnológica e Ambiental Escola de Química e AlimentosRio Grande RS ,Brazil

关键词: pesticides;    groundwater;    HPLC-DAD;    SPE;    validation;   

DOI  :  10.1590/S0103-50532009000100020

来源: SciELO

【 摘 要 】

A method is described for the determination of the pesticides carbofuran, clomazone, 2,4-D and tebuconazole in groundwaters. The method involves solid phase extraction (SPE) with C18 cartridges and quantification by high performance liquid chromatography with diode array detector (HPLC-DAD). After the optimization of the extraction and separation parameters, the method was validated by evaluating the analytical curve, linearity, limits of detection and quantification, precision and accuracy (recovery). The method presents an average recovery of 87.9% and 96.9%, in repeatability and intermediate precision conditions, respectively, with adequate precision (RSD from 0.8 to 20.7%), for all compounds. The method will be applied to determine pesticides in groundwater samples with limit of quantification of 0.2 μg L-1.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License