Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs

Journal of the Brazilian Chemical Society

Carolina V. Uliana¹  Carla S. Riccardi¹  João O. Tognolli¹  Hideko Yamanaka¹ 

[1] ,Universidade Estadual Paulista Instituto de Química Araraquara SP ,Brazil

关键词: biosensor;    hepatitis C virus;    fractional factorial design;   

DOI  :  10.1590/S0103-50532008000400024

来源: SciELO

【 摘 要 】

Fractional factorial design and factorial with center point design were applied to the development of an amperometric biosensor for the detection of the hepatitis C virus. Biomolecules were immobilized by adsorption on graphite electrodes modified with siloxane-poly(propyleneoxide) hybrid matrix prepared using the sol-gel method. Several parameters were optimized, such as the streptavidin concentration at 0.01 mg mL-1 and 1.0% bovine serum albumin, the incubation time of the electrodes in the complementary DNA solution for 30 minutes and a 1:1500 dilution of the avidin-peroxidase conjugate, among others. The application of chemometric studies has been efficient, since the best conditions have been established with a restricted number of experiments, indicating the influence of different factors on the system.

【 授权许可】

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