Journal of the Brazilian Chemical Society | |
Optimization of an amperometric biosensor for the detection of hepatitis C virus using fractional factorial designs | |
Tognolli, João O.1  Yamanaka, Hideko1  Uliana, Carolina V.1  Riccardi, Carla S.1  Universidade Estadual Paulista, Araraquara, Brazil1  | |
关键词: biosensor; hepatitis C virus; fractional factorial design; | |
DOI : 10.1590/S0103-50532008000400024 | |
学科分类:化学(综合) | |
来源: SciELO | |
【 摘 要 】
Fractional factorial design and factorial with center point design were applied to the development of an amperometric biosensor for the detection of the hepatitis C virus. Biomolecules were immobilized by adsorption on graphite electrodes modified with siloxane-poly(propyleneoxide) hybrid matrix prepared using the sol-gel method. Several parameters were optimized, such as the streptavidin concentration at 0.01 mg mL-1 and 1.0% bovine serum albumin, the incubation time of the electrodes in the complementary DNA solution for 30 minutes and a 1:1500 dilution of the avidin-peroxidase conjugate, among others. The application of chemometric studies has been efficient, since the best conditions have been established with a restricted number of experiments, indicating the influence of different factors on the system.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912050580424ZK.pdf | 180KB | download |