Flavonoids from Annona dioica leaves and their effects in Ehrlich carcinoma cells, DNA-topoisomerase I and II

Journal of the Brazilian Chemical Society

Maria R. G. Vega²  Andressa Esteves-souza²  Ivo J. C. Vieira¹  Leda Mathias¹  Raimundo Braz-filho¹  Aurea Echevarria² 

[1] ,Universidade Federal Rural do Rio de Janeiro Departamento de Química Seropédica RJ ,Brazil

关键词: Annona dioica;    Annonaceae;    cytotoxic activity;    flavonoids;    DNA-topoisomerase;   

DOI  :  10.1590/S0103-50532007000800016

来源: SciELO

【 摘 要 】

Chemical investigation of methanol extract leaves from Annona dioica (Annonaceae) resulted in the identification of flavonoids kaempferol (1), 3-O-[3",6"-di-O-p-hydroxycinnamoyl]-beta-galactopyranosyl-kaempferol (2), 6"-O-p-hydroxycinnamoyl-beta-galactopyranosyl-kaempferol (3) and 3-O-beta-galactopyranosyl-kaempferol (4). The structures were unequivocally characterized by ¹H and 13C NMR spectroscopic analyses using 1D and 2D experiments. The cytotoxic effects of the flavonoids and flavonoid fraction (FF) were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay against Ehrlich carcinoma cells. The results indicated that 1, 2, 3 and FF exhibit significant antiproliferative action when compared to quercetin. The inhibitory action on DNA-topoisomerase I and II of all the flavonoids was evaluated by relaxation assays on pBR322 plasmid DNA. The results indicated the inhibitory and non-selective effects of the flavonoids on DNA-topoisomerase I and II.

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