Flavonoids from Annona dioica leaves and their effects in Ehrlich carcinoma cells, DNA-topoisomerase I and II

Journal of the Brazilian Chemical Society

Braz-Filho, Raimundo¹  Esteves-Souza, Andressa¹  Mathias, Leda¹  Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, Brazil¹  Vieira, Ivo J. C.¹  Echevarria, Aurea¹  Universidade Federal Rural do Rio de Janeiro, Seropédica, Brazil¹  Vega, Maria R. G.¹ 

关键词: Annona dioica;    Annonaceae;    cytotoxic activity;    flavonoids;    DNA-topoisomerase;   

DOI  :  10.1590/S0103-50532007000800016

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Chemical investigation of methanol extract leaves from Annona dioica (Annonaceae) resulted in the identification of flavonoids kaempferol (1), 3-O-[3",6"-di-O-p-hydroxycinnamoyl]-b-galactopyranosyl-kaempferol (2), 6"-O-p-hydroxycinnamoyl-b-galactopyranosyl-kaempferol (3) and 3-O-b-galactopyranosyl-kaempferol (4). The structures were unequivocally characterized by 1H and 13C NMR spectroscopic analyses using 1D and 2D experiments. The cytotoxic effects of the flavonoids and flavonoid fraction (FF) were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay against Ehrlich carcinoma cells. The results indicated that 1, 2, 3 and FF exhibit significant antiproliferative action when compared to quercetin. The inhibitory action on DNA-topoisomerase I and II of all the flavonoids was evaluated by relaxation assays on pBR322 plasmid DNA. The results indicated the inhibitory and non-selective effects of the flavonoids on DNA-topoisomerase I and II.

【 授权许可】

Unknown