HPLC analysis of glycosylated amino acids

Journal of the Brazilian Chemical Society

Vanessa L. Campo¹  Áurea D. L. Borges¹  Ivone Carvalho¹ 

[1] ,Universidade de São Paulo Faculdade de Ciências Farmacêuticas de Ribeirão Preto Departamento de Ciências FarmacêuticasRibeirão Preto SP ,Brazil

关键词: glycosylated amino acids;    glycosylation reaction;    HPLC;   

DOI  :  10.1590/S0103-50532006000400004

来源: SciELO

【 摘 要 】

This work describes the standardization of HPLC analysis methodology for the identification and separation of the glycosylated amino acids (building blocks) 3, 4, 7 and 8 and their respective precursors. The glycosylated amino acids containing the sugar units beta-D-GlcNAc (3 and 7) O-linked to threonine amino acids are involved in cellular signalling events, and the correspondent isomers 4 and 8, possessing alpha-D-GlcNAc units are related to O-glycans found in mucins of the parasite Trypanosoma cruzi. The developed methodology is advantageous and efficient as it permits the analysis of crude reaction mixtures, avoiding exhaustive purification processes.

【 授权许可】

CC BY   
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