Journal of the Brazilian Chemical Society | |
HPLC analysis of glycosylated amino acids | |
Universidade de São Paulo, Ribeirão Preto, Brazil1  Borges, Áurea D. L.1  Campo, Vanessa L.1  Carvalho, Ivone1  | |
关键词: glycosylated amino acids; glycosylation reaction; HPLC; | |
DOI : 10.1590/S0103-50532006000400004 | |
学科分类:化学(综合) | |
来源: SciELO | |
【 摘 要 】
This work describes the standardization of HPLC analysis methodology for the identification and separation of the glycosylated amino acids (building blocks) 3, 4, 7 and 8 and their respective precursors. The glycosylated amino acids containing the sugar units b-D-GlcNAc (3 and 7) O-linked to threonine amino acids are involved in cellular signalling events, and the correspondent isomers 4 and 8, possessing a-D-GlcNAc units are related to O-glycans found in mucins of the parasite Trypanosoma cruzi. The developed methodology is advantageous and efficient as it permits the analysis of crude reaction mixtures, avoiding exhaustive purification processes.
【 授权许可】
Unknown
【 预 览 】
Files | Size | Format | View |
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RO201912050579956ZK.pdf | 700KB | download |