Journal of the Brazilian Chemical Society | |
Boron isotope dilution in cellular fractions of coffee leaves Evaluated by Inductively Coupled Plasma Mass Spectrometry with direct injection nebulization (DIN-ICP-MS) | |
Ana Cláudia S. Bellato1  Amauri A. Menegário1  Maria Fernanda Giné1  | |
[1] ,Universidade de São Paulo Centro de Energia Nuclear na Agricultura Piracicaba SP ,Brazil | |
关键词: isotope dilution; inductively coupled plasma mass spectrometry; biological cell fractionation; boron isotope ratios; plant analysis; | |
DOI : 10.1590/S0103-50532003000200014 | |
来源: SciELO | |
【 摘 要 】
Enriched 10B (94.14 atom %) was supplied to coffee plantlets for three months. Then boron isotope ratios were determined in the leaf cell compartments, cell wall, nuclei and chloroplast, after a sub-cellular fractionation procedure. The isotopic measurements were performed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) provided with a direct injection nebulizer (DIN), introducing a sample volume of 50 mL. Isotopic ratios from 1.002 to 1.326 were determined with precision characterized by RSD lower than 1.5% for the enriched cell fractions with B concentrations ranging from 3.3 to 10.8 mg g-1. The detection limit (3s) was 0.5 ng B mL-1. The average enrichments in 10B atom % found in the cell walls, nuclei and chloroplasts were 46.7, 44.5 and 48.8, respectively.
【 授权许可】
CC BY
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