Boron isotope dilution in cellular fractions of coffee leaves Evaluated by Inductively Coupled Plasma Mass Spectrometry with direct injection nebulization (DIN-ICP-MS)

Journal of the Brazilian Chemical Society

Menegário, Amauri A.¹  Universidade de São Paulo, Piracicaba, Brazil¹  Bellato, Ana Cláudia S.¹  Giné, Maria Fernanda¹ 

关键词: isotope dilution;    inductively coupled plasma mass spectrometry;    biological cell fractionation;    boron isotope ratios;    plant analysis;   

DOI  :  10.1590/S0103-50532003000200014

学科分类：化学（综合）

来源: SciELO

【 摘 要 】

Enriched 10B (94.14 atom %) was supplied to coffee plantlets for three months. Then boron isotope ratios were determined in the leaf cell compartments, cell wall, nuclei and chloroplast, after a sub-cellular fractionation procedure. The isotopic measurements were performed by Inductively Coupled Plasma Mass Spectrometry (ICP-MS) provided with a direct injection nebulizer (DIN), introducing a sample volume of 50 mL. Isotopic ratios from 1.002 to 1.326 were determined with precision characterized by RSD lower than 1.5% for the enriched cell fractions with B concentrations ranging from 3.3 to 10.8 mg g-1. The detection limit (3s) was 0.5 ng B mL-1. The average enrichments in 10B atom % found in the cell walls, nuclei and chloroplasts were 46.7, 44.5 and 48.8, respectively.

【 授权许可】

Unknown