Importance of duration and number of ischemic postconditioning cycles in preventing reperfusion mesenteric injuries. Experimental study in rats

Acta Cirurgica Brasileira

Carlos Henrique Marques Dos Santos¹  Ricardo Dutra Aydos¹  Ed Nogueira Neto¹  Luciana Nakao Odashiro Miiji¹  Pedro Carvalho Cassino¹  Isadora Ishaq Ahmed¹  Nadia Meneguesso Calheiros¹  Milena Garcia¹  Anderson Fernandes Da Silva¹ 

关键词: Ischemic Postconditioning;    Ischemia;    Reperfusion;    Intestines;    Rats;   

DOI  :  10.1590/S0102-865020150100000009

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the effect of ischemic postconditioning (IPC) on intestinal mucosa of rats subjected to ischemia and reperfusion process comparing two cycles of reperfusion and ischemia lasting two minutes each and four cycles of reperfusion and ischemia lasting 30 seconds eachMETHODS:Thirty Wistar rats were distributed into three groups: group A (10 rats), ischemia (30 minutes) and reperfusion (60 minutes); group B (10 rats), ischemia and reperfusion plus IPC by two lasting two minutes each; and Group C (10 rats), ischemia and reperfusion plus IPC by four cycles lasting 30 seconds each. Finally, a segment of small intestine was resected for histological analysis. We analysed the results according to Chiu et al . classification and proceeded to the statistical treatment by Kruskal-Wallis test (p<0.05).RESULTS:The mean degree of tissue injury according to Chiu et al . classification were: Group A, 2.77; in group B, 1.4; and group C, 1.4. B X C (p<0.05).CONCLUSIONS:Ischemic postconditioning was able to minimize reperfusion injury of rats undergone mesenteric ischemia and reperfusion process. There was no difference in the effectiveness of the method comparing two cycles of two minutes with four cycles of 30 seconds by H&E histological evaluation of the ileum after 60-minute reperfusion.

【 授权许可】

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