Isolation and characterization of mesenchymal progenitors derived from the bone marrow of goats native from northeastern Brazil

Acta Cirurgica Brasileira

Osmar Ferreira Da Silva Filho¹  Napoleão Martins Argôlo Neto¹  Maria Acelina Martins De Carvalho¹  Yulla Klinger De Carvalho¹  Anaemilia Das Neves Diniz¹  Laécio Da Silva Moura¹  Carlos Eduardo Ambrósio¹  Janaína Munuera Monteiro¹  Hatawa Melo De Almeida¹  Maria Angélica Miglino¹  Jacyara De Jesus Rosa Pereira Alves¹  Kássio Vieira Macedo¹  Andressa Rego Da Rocha¹  Matheus Levi Tajra Feitosa¹  Flávio Ribeiro Alves¹ 

关键词: Cell Culture Techniques;    Cell Differentiation;    Stem Cells;    Flow Cytometry;    Goats;   

DOI  :  10.1590/S0102-86502014000800001

来源: SciELO

【 摘 要 】

PURPOSE:To characterize bone marrow progenitors cells grown in vitro,using native goats from northeastern Brazil as animal model.METHODS:Ten northeastern Brazil native goats of both genders were used from the Piauí Federal University Agricultural Science Center's (UFPI) - Goat Farming Sector. Bone marrow aspirates where taken from the tibial ridge and seeded on culture plates for isolation, expansion and Flow Cytometry (expression markers - Oct-3/4, PCNA, Ck-Pan, Vimentina, Nanog).RESULTS:Progenitor cells showed colonies characterized by the presence of cell pellets with fibroblastoid morphology. Cell confluence was taken after 14 days culture and the non-adherent mononuclear cell progressive reduction. After the first passage, 94.36% cell viability was observed, starting from 4.6 x 106 cell/mL initially seeded. Cells that went through flow cytometry showed positive expression for Oct-3/4, PCNA, Ck-Pan, Vimentina, and Nanog.CONCLUSIONS:Bone marrow progenitor isolated of native goats from northeastern Brazil showed expression markers also seen in embryonic stem cells (Oct-3/4, Nanog), markers of cell proliferation (PCNA) and markers for mesenchymal cells (Vimentina and Ck-pan), which associated to morphological and culture growth features, suggest the existence of a mesenchymal stem cell (MSC) population in the goat bone marrow stromal cells studied.

【 授权许可】

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