Gene expression profile of oxidative stress in the lung of inbred mice after intestinal ischemia/reperfusion injury

Acta Cirurgica Brasileira

Adauto Tsutomu Ikejiri¹  Frederico Somaio Neto¹  José Carlos Chaves¹  Paulo Roberto Bertoletto¹  Roberto Teruya¹  Eduardo Rodrigues Bertoletto¹  Murched Omar Taha¹  Djalma José Fagundes¹ 

关键词: Gene Expression;    Oxidative Stress;    Lung;    Reperfusion Injury;    Mice;   

DOI  :  10.1590/S0102-86502014000300007

来源: SciELO

【 摘 要 】

PURPOSE: To determine the gene expression profile associated with oxidative stress and antioxidant defense in the lung tissue of mice subjected to intestinal ischemia and reperfusion.METHODS: Twelve male, inbred mice (C57BL/6) were randomly assigned to one of two groups. The control group (CG) underwent anesthesia and laparotomy and was observed for 120 minutes; the ischemia/reperfusion group (IRG) was subjected to anesthesia, laparotomy, and ischemia of the small intestine for 60 minutes and to 60 minutes of reperfusion. A pool of six mice from each group was subjected to a reverse transcription-quantitative polymerase chain reaction (RT-qPCR) to analyze the oxidative stress and antioxidant defense genes. All genes that were up-regulated or down-regulated greater than three-fold, based on the algorithm [2

【 授权许可】

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