Effect of nitrofen in the final stages of development of the diaphragm muscle in rats

Acta Cirurgica Brasileira

Frances Lilian Lanhellas Gonçalves²  Fábio Santana De Oliveira¹  Augusto Frederico Schmidt¹  Luís Antônio Violin Dias Pereira¹  Rodrigo Melo Gallindo²  Rebeca Lopes Figueira²  Lourenço Sbragia² 

[1] ,University of Sao Paulo School of Medicine of Ribeirao Preto Department of Surgery and AnatomyRibeirao Preto SP ,Brazil

关键词: Hernia;    Diaphragmatic;    Diaphragm;    Abnormalities;    Drug-Induced;    Myosins;    Rats;    Hérnia Diafragmática;    Diafragma;    Anormalidades Induzidas por Medicamentos;    Miosinas;    Ratos;   

DOI  :  10.1590/S0102-86502013001300004

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the expression of myosin in muscle fibers of the diaphragm in experimental congenital diaphragmatic hernia (CDH). METHODS: Fetuses of pregnant rats were divided into four groups: External Control (EC), composed of non-manipulated rats; Nitrofen, composed of pregnant rats that received 100 mg of nitrofen (2,4-dichloro-4'nitrodiphenyl ether) diluted in olive oil on gestational day (GD) 9.5, whose fetuses developed CDH (N+) or not (N-), and Olive Oil Placebo (OO), composed of pregnant rats that received the oil on the same GD. The fetuses were collected on GD 18.5, 19.5, 20.5 and 21.5 (term = 22 days). We obtained body weight (BW) and photographed the diaphragm area (DA), hernia area (HA) and subsequent calculated the HA/DA ratio in N+ group. Samples of Diaphragm muscle were processed for histological staining with H/E and immunohistochemistry (IHQ) for myosin.} RESULTS: The fetuses of N- and N+ groups had decreased BW and DA compared to EC and OO groups (p <0.001). HA was decreased on GD 18.5 compared to 21.5 (p <0.001) and the HA/DA ratio showed no difference. IHQ showed decreased expression of myosin in nitrofen groups. CONCLUSION: CDH induced by nitrofen model contributes to the understanding of muscularization in the formation of the diaphragm where the myosin expression is decreased.

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