Role of protein kinase G on the post-shock mesenteric lymph blockage ameliorating vascular calcium sensitivity

Acta Cirurgica Brasileira

Zi-gang Zhao¹  Yan-ling Wei¹  Chun-yu Niu¹  Yu-ping Zhang¹  Li-min Zhang¹  Li-na Jiang¹ 

[1] ,Hebei North University Institute of Microcirculation Zhangjiakou,China

关键词: Shock;    Hemorrhagic;    Protein Kinases;    Calcium;    Lymph;    Rats;   

DOI  :  10.1590/S0102-86502013000700010

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the role of protein kinase G (PKG) in blocking post-shock mesenteric lymph (PSML) return ameliorating the calcium sensitivity in hemorrhagic shock rats. METHODS: Male Wistar rats were randomly divided into sham, shock, shock+ligation (shock plus mesenteric lymph duct ligation (MLDL)), shock+drainage (shock plus PSML drainage) groups. After shock (hypotension 40mmHg) for three hours or corresponding times, the superior mesenteric artery (SMA) was taken out for detecting the PKG and phospho PKG (p-PKG) contents, and the vascular rings of SMA were prepared for assaying the calcium sensitivity using an isolated organ perfusion system. RESULTS: The PKG and p-PKG contents of SMA in shock group were significantly increased than that of sham group, and MLDL or PSML drainage reducing the levels of PKG and p-PKG. Meanwhile, the vascular calcium sensitivity in shock group was significantly lower than that of sham group, MLDL or PSML drainage enhanced the calcium sensitivity. After incubating with PKG regulators in shock+ligation and shock+drainage groups, the PKG agonist 8Br-cGMP reduced the contractility of vascular rings to gradient calcium ions and Emax and the PKG inhibitor agonist KT5823 elevated the calcium sensitivity significantly. CONCLUSION: Protein kinase G plays an important role in post-shock mesenteric lymph blockage improving vascular calcium sensitivity.

【 授权许可】

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