Hyperbaric oxygen therapy and ischemia and reperfusion: a valuable association to attenuate ischemic lesion and hepatic reperfusion

Acta Cirurgica Brasileira

Daniele Moraes Losada¹  Maria Eliza Jordani De Souza¹  Maria Cecília Jordani¹  Maria Aparecida Neves Cardoso Picinato¹  Clarice Fleury Fina¹  Omar Feres¹  Paulo Roberto Teixeira Michelone²  Orlando De Castro E Silva¹ 

[1] ,FAMEMA Surgery Department Marilia SP ,Brazil

关键词: Hyperbaric Oxygenation;    Ischemia;    Reperfusion;    Liver;    Rats;   

DOI  :  10.1590/S0102-86502013000200007

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the consequences of the association between hyperbaric oxygen therapy and hepatic ischemia / reperfusion. METHODS: Wistar rats were divided into three groups: SHAM, rats submitted to surgical stress and anesthetic but not hepatic ischemia or reperfusion, I / R, rats submitted to total hepatic pedicle ischemia for 30 min, followed by 5 min of reperfusion; HBO120, rats submitted to 120 min of hyperbaric oxygen therapy at two absolute atmospheres and immediately after submitted to the experimental protocol of ischemia and reperfusion. The preservation of the hepatic function was evaluated by determining mitochondrial swelling and malondialdehyde tissue level, as well as alanine aminotransferase and aspartate aminotranferase serum levels. The results were analyzed using the Mann-Whitney test and differences were considered significant for p<0.05. RESULTS: There were significant differences in values: mitochondrial swelling of the I / R group compared to SHAM and HBO120; malondialdehyde between SHAM vs. I / R, SHAM vs HBO120, and I / R vs HBO120, alanine aminotransferase between SHAM vs. I / R . There was no significant difference between groups in aspartate aminotransferase serum levels. CONCLUSION: The association between hyperbaric oxygen therapy and hepatic ischemia and reperfusion process was positive.

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