Sciatic nerve regeneration in rats by a nerve conduit engineering with a membrane derived from natural latex

Acta Cirurgica Brasileira

Marcos Vinícius Muniz Ganga¹  Joaquim Coutinho-netto²  Benedicto Oscar Colli¹  Wilson Marques Junior¹  Carlos Henrique Rocha Catalão¹  Ricardo Torres Santana¹  Marcos Roberto Pedron Oltramari¹  Kleber Tadeu Carraro¹  João-josé Lachat¹  Luiza Da Silva Lopes¹ 

[1] ,USP School of Medicine of Ribeirao Preto Department of Biochemistry and ImmunologyRibeirao Preto SP ,Brazil

关键词: Nerve Regeneration;    Latex;    Tissue Engineering;    Rats;    Regeneração Nervosa;    Látex;    Engenharia Tecidual;    Ratos;   

DOI  :  10.1590/S0102-86502012001200010

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the capacity of natural latex membrane to accelerate and improve the regeneration quality of the of rat sciatic nerves. METHODS: Forty male adult Wistar rats were used, anesthetized and operated to cut the sciatic nerve and receive an autograft or a conduit made with a membrane derived from natural latex (Hevea brasiliensis). Four or eight weeks after surgery, to investigate motor nerve recovery, we analyzed the neurological function by walking pattern (footprints analysis and computerized treadmill), electrophysiological evaluation and histological analysis of regenerated nerve (autologous nerve graft or tissue cables between the nerve stumps), and anterior tibial and gastrocnemius muscles. RESULTS: All functional and morphological analysis showed that the rats transplanted with latex conduit had a better neurological recovery than those operated with autologous nerve: quality of footprints, performance on treadmill (p<0.01), electrophysiological response (p<0.05), and quality of histological aspects on neural regeneration. CONCLUSION: The data reported showed behavioral and functional recovery in rats implanted with latex conduit for sciatic nerve repair, supporting a complete morphological and physiological regeneration of the nerve.

【 授权许可】

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