Association of mesenchymal stem cells with platelet rich plasma on the repair of critical calvarial defects in mice

Acta Cirurgica Brasileira

Betânia Souza Monteiro²  Ricardo Junqueira Del Carlo¹  Napoleão Martins Argôlo-neto¹  Nance Beyer Nardi¹  Pablo Herthel Carvalho¹  Laila De Paula Bonfá¹  Pedro César Chagastelles¹  Higo Nasser Moreira¹  Marlene Isabel Vargas Viloria¹  Bianka Souza Dos Santos¹ 

[1] ,Vila Velha University Department of VeterinaryEspirito Santo ,Brazil

关键词: Mesenchymal Stem Cells;    Platelet-Rich Plasma;    Tissue Therapy;    Skull;    Mice;    Células-Tronco Mesenquimais;    Plasma Rico em Plaquetas;    Terapia Tecidual;    Crânio;    Camundongos;   

DOI  :  10.1590/S0102-86502012000300001

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the effects of mesenchymal stem cells (MSC) from eight mice C57BL/6 gfp+ bone marrows expanded in cultures associated with platelets rich plasma (PRP) deriving from another eight mice, in the repair of critical defects in calvarial bone produced in twenty-four adult isogenic mice C57BL/6. METHODS: The animals were submitted to a cranial defect of 6.0mm in diameter and divided into two equal experimental groups. Control group did not receive treatment and the treated group received a MSC pellet containing 1.0 x 10(7) cells/mL associated with 50.0µL of plasma gel containing 1.0 x 10(9) autologous platelets within the defect. RESULTS: In the treated group was observed process of angiogenesis and bone repair better than control group. CONCLUSION: Mesenchymal stem cells derived from bone marrow of C57BL/6 gfp+ mice associated with PRP gel applied in bone critical defects produced in calvarial contributes positively to the process of bone repair.

【 授权许可】

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