Effects of controlled and pressure support mechanical ventilation on rat diaphragm muscle

Acta Cirurgica Brasileira

André De Sá Braga Oliveira²  Lívia Bandeira Costa¹  Thiago De Oliveira Assis¹  Diógenes Luís Da Mota¹  Eduardo Ériko Tenório De França¹  José Cândido De Araújo Filho¹  Silvania Tavares Paz Rosas¹  Paloma Lys De Medeiros¹ 

[1] ,Integrated Colleges of Patos,Brazil

关键词: Anatomy & Histology;    Diaphragm;    Tracheostomy;    Rats;    Wistar;    Anatomia & Histologia;    Diafragma;    Traqueostomia;    Ratos Wistar;   

DOI  :  10.1590/S0102-86502012000200003

来源: SciELO

【 摘 要 】

PURPOSE: The objective of this study was to analyze the effects of Pressure Controlled Ventilation mode (PCV-C) and PSV mode in diaphragm muscle of rats. METHODS: Wistar rats (n=18) were randomly assigned to the control group or to receive 6 hours of PCV and PSV. After this period, animals were euthanized and their diaphragms were excised, frozen in liquid nitrogen and stored in at -80º C for further histomorphometric analysis. RESULTS: Results showed a 15% decrease in cross-sectional area of muscle fibers on the PCV-C group when compared to the control group (p<0.001) and by 10% when compared to the PSV group (p<0.05). Minor diameter was decreased in PCV-C group by 9% when compared with the control group (p<0.001) and by 6% when compared to the PSV group (p<0.05). When myonuclear area was analyzed, a 16% decrease was observed in the PCV-C group when compared to the PSV group (p<0.05). No significant difference between the groups was observed in myonuclear perimeter (p>0.05). CONCLUSION: Short-term controlled mechanical ventilation seems to lead to muscular atrophy in diaphragm fibers. The PSV mode may attenuate the effects of VIDD.

【 授权许可】

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