Morphometric analysis of dendritic cells from anal mucosa of HIV-positive patients and the relation to intraepithelial lesions and cancer seen at a tertiary health institution in Brazil

Acta Cirurgica Brasileira

Adriana Gonçalves Daumas Pinheiro Guimarães²  Roberto Moreira Da Silva Junior¹  Oscar Tadeu Ferreira Da Costa¹  Ivan Tramujas Da Costa E Silva²  Felicidad Santos Gimenez¹  José Ribamar De Araujo¹  Rosilene Viana De Andrade¹  Erico Jander Da Silva Lopes¹  Jacqueline Pereira Pinheiro¹  Junia Raquel D. Ferreira²  Adriana Malheiro¹  Luiz Carlos De Lima Ferreira² 

[1] ,UFAMManaus AM ,Brazil

关键词: Dendritic Cells;    HIV;    Anus Diseases;    Neoplasms;    Células Dendríticas;    HIV;    Doenças do Ânus;    Neoplasias;   

DOI  :  10.1590/S0102-86502011000600019

来源: SciELO

【 摘 要 】

PURPOSE: To morphometrically quantify CD1a+ dentritic cells and DC-SIGN+ dendritic cells in HIV-positive patients with anal squamous intraepithelial neoplasia and to evaluate the effects of HIV infection, antiretroviral therapy and HPV infection on epithelial and subepithelial dendritic cells. METHODS: A prospective study was performed to morphometrically analyze the relative volume of the dendritic cells and the relationship between anal intraepithelial neoplasia and cancer in HIV-positive patients from the Tropical Medicine Foundation of Amazonas, Brazil. All patients were submitted to biopsies of anorectal mucosa to perform a classic histopathological and immunohistochemical analysis, employing antibodies against CD1a and DC-SIGN for the morphometric quantification of dendritic cells. RESULTS: HIV-negative patients displayed a CD1a DC density significantly higher than that of HIV-positives patients (3.75 versus 2.54) (p=0.018), and in patients with severe anal intraepithelial neoplasia had correlated between DC CD1a density with levels of CD4 + cells (p: 0.04) as well as the viral load of HIV-1 (p: 0.035). A not significant rise in the median density of CD1a+ DC was observed in the HIV positive/ HAART positive subgroup compared to the HIV positive/ HAART negative subgroup. The CD1a+ DC were also significantly increased in HIV-negative patients with anorectal condyloma (2.33 to 3.53; p=0.05), with an opposite effect in HIV-positive patients. CONCLUSIONS: Our data support an enhancement of the synergistic action caused by HIV-HPV co-infection on the anal epithelium, weakening the DC for its major role in immune surveillance. Notoriously in patients with severe anal intraepithelial neoplasia, the density of CD1a+ epithelial dendritic cells was influenced by the viral load of HIV-1. Our study describes for the first time the density of subepithelial DC-SIGN+ dendritic cells in patients with anal severe anal intraepithelial neoplasia and points to the possibility that a specific therapy for HIV induces the recovery of the density of epithelial DC.

【 授权许可】

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