Small bowel transplantation in outbred rats

Acta Cirurgica Brasileira

Daniel Reis Waisberg²  André Dong Wong Lee¹  Rafael Miyashiro Nunes Dos Santos¹  Eduardo Kenji Mory¹  Anderson Lino Costa¹  Edna Frasson De Souza Montero¹  Eleazar Chaib¹  Luis Augusto Carneiro D'albuquerque¹  Flavio Henrique Ferreira Galvao¹ 

[1] ,USP FM Sao Paulo,Brazil

关键词: Intestine;    Small;    Transplantation;    Microsurgery;    Animals;    Outbred Strains;    Rats;    Intestino Delgado;    Transplante;    Microcirurgia;    Animais não Endogâmicos;    Ratos;   

DOI  :  10.1590/S0102-86502011000600015

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the clinical evolution of orthotopic small bowel transplantation in outbred rats. METHODS: Seventy-two outbred Wistar rats weighting from 250 to 300g were used as donor and recipient in 36 consecutives ortothopic small intestine transplantation without immunosuppression. The graft was transplanted into the recipient using end-to-side aortic and portacaval microvascular anastomosis. Procedure duration, animal clinical course and survival were evaluated. Survival shorter than four days was considered technical failure. Recipients were sacrificed with signs of severe graft rejection or survival longer than 120 days. Necropsies were performed in all recipients to access histopathological changes in the graft. RESULTS: Median time for the procedure was 107 minutes. Six recipients (16.7%) presented technical failure. Twenty-seven recipients were sacrificed due to rejection, being nineteen (52.7%) between 7th and 15th postoperative day and eight (22.2%) between 34th and 47th postoperative day. Graft histology confirmed severe acute cellular rejection in those recipients. Uneventful evolution and survival longer than 120 days without rejection were observed in three recipients (8.3%). CONCLUSION: Intestinal transplantation in outbred rats without immunosuppressant regiment accomplishes variable clinical evolution.

【 授权许可】

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