Comparative study of technique to obtain stem cells from bone marrow collection between the iliac crest and the femoral epiphysis in rabbits

Acta Cirurgica Brasileira

Lilian Piñero Eça²  Renata Belmonte Ramalho¹  Isis Sousa Oliveira¹  Paulo Oliveira Gomes¹  Paulo Pontes¹  Alice Teixeira Ferreira¹  Marcelo Paulo Vaccari Mazzetti¹ 

[1] ,Stem Cell Research InstituteSao Paulo,Brazil

关键词: Bone Marrow;    Adult Stem Cells;    Rabbits;    Medula Óssea;    Células-Tronco Adultas;    Coelhos;   

DOI  :  10.1590/S0102-86502009000500011

来源: SciELO

【 摘 要 】

PURPOSE: To assess the technique for the collection of rabbit bone marrow stem cells from different regions to be used as an experimental model in regenerative medicine. METHODS: Thirty rabbits were allocated into 2 groups: GROUP A, n=8, animals that underwent bone marrow blood (BMB) harvesting from the iliac crest; and GROUP B: including 22 rabbits that underwent BMB harvesting from the femur epiphysis. After harvesting, mononuclear cells were isolated by density gradient centrifugation (Ficoll - Histopaque). The number of mononuclear cells per ml was counted in a Neubauer chamber and cell viability was checked through Tripan Blue method. RESULTS: Harvesting from the iliac crest yielded an average of 1 ml of BMB and 3,6.10(6) cells/ml over 1 hour of surgery, whereas an average of 3ml of BMB and 11,79.10(6) cells./ml were obtained in 30 min from the femur epiphysis with a reduced animal death rate. CONCLUSION: The analysis for the obtention of a larger number of mononuclear cells/ml from rabbit bone marrow blood was more satisfactory in the femur epiphysis than in the iliac crest.

【 授权许可】

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