Cell Cycle Related Differentiation of Bone Marrow Cells into Lung Cells

Dooner, Mark ; Aliotta, Jason M. ; Pimental, Jeffrey ; Dooner, Gerri J. ; Abedi, Mehrdad ; Colvin, Gerald ; Liu, Qin ; Weier, Heinz-Ulli ; Dooner, Mark S. ; Quesenberry, Peter J.

Lawrence Berkeley National Laboratory

关键词: Respiratory Tract Cells;    Steels;    Lungs;    Proteins;    Bone Marrow;   

DOI  :  10.2172/936517
RP-ID  :  LBNL-842E
RP-ID  :  DE-AC02-05CH11231
RP-ID  :  936517

美国|英语

来源: UNT Digital Library

【 摘 要 】

Green-fluorescent protein (GFP) labeled marrow cells transplanted into lethally irradiated mice can be detected in the lungs of transplanted mice and have been shown to express lung specific proteins while lacking the expression of hematopoietic markers. We have studied marrow cells induced to transit cell cycle by exposure to IL-3, IL-6, IL-11 and steel factor at different times of culture corresponding to different phases of cell cycle. We have found that marrow cells at the G1/S interface have a 3-fold increase in cells which assume a lung phenotype and that this increase is no longer seen in late S/G2. These cells have been characterized as GFP{sup +} CD45{sup -} and GFP{sup +} cytokeratin{sup +}. Thus marrow cells with the capacity to convert into cells with a lung phenotype after transplantation show a reversible increase with cytokine induced cell cycle transit. Previous studies have shown the phenotype of bone marrow stem cells fluctuates reversibly as these cells traverse cell cycle, leading to a continuum model of stem cell regulation. The present studies indicate that marrow stem cell production of nonhematopoietic cells also fluctuates on a continuum.