Acta Cirurgica Brasileira | |
Analysis of the bactericidal effect of ozone pneumoperitoneum | |
Rodrigo Altenfelder Silva2  José Eduardo Rosseto Garotti1  Renata Santos Bittencourt Silva1  Alessandra Navarini1  Adhemar Monteiro Pacheco Jr2  | |
[1] ,Faculty of Medical Sciences Santa Casa de Sao Paulo Sao Paulo,Brazil | |
关键词: Peritonitis; Ozone; Laparoscopy; Peritoneum; Rats; Peritonite; Ozônio; Laparoscopia; Peritônio; Ratos; | |
DOI : 10.1590/S0102-86502009000200009 | |
来源: SciELO | |
【 摘 要 】
PURPOSE: To assess the bactericidal action of ozone pneumoperitonium, and to compare the results with CO2. METHODS: It was used 36 Wistar rats. The animals, under anesthesia, were inoculated with 2ml of E. coli ATCC at a concentration of 10(10)UFC, and 1ml of BaSO4, into the peritoneal cavity. They were divided into three groups: Group 1, CO2 pneumoperitoneum was performed for 15 minutes; Group 2, ozone pneumoperitoneum was performed for 5 minutes at a concentration of 42µg/ml, and Group 3, ozone pneumoperitoneum was performed for 5 minutes at a concentration of 62µg/ml. Six animals from each group were sacrificed after the experiment, and the remaining 6 observed for 24 hours. Material was collected from the cavity of all animals for microbiological study. RESULTS: Ozone presented a greater bactericidal effect than CO2 in those animals sacrificed immediately after pneumoperitoneum. In the animals studied 24 hours after pneumoperitoneum evidenced no difference in bactericidal effect between the two gases. Moreover, no difference in mortality was observed. CONCLUSION: Ozone has a more potent bactericidal effect than carbon dioxide gas, although this did not influence survival of the animals.
【 授权许可】
CC BY
All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License
【 预 览 】
Files | Size | Format | View |
---|---|---|---|
RO202005130100343ZK.pdf | 142KB | download |