The influence of estrogen on liver regeneration: an experimental study in rats

Acta Cirurgica Brasileira

Maria De Lourdes Pessole Biondo-simões²  Thomas Rolf Erdmann¹  Sérgio Ossamu Ioshii¹  Jorge Eduardo Fouto Matias¹  Hugo Leonardo Guaita Calixto¹  Diego José Schebelski¹ 

[1] ,Pontific Catholic University of Parana UFPR and Research Methods Department of Surgery,Brazil

关键词: Liver Regeneration;    Liver;    Estradiol;    Hepatectomy;    Regeneração Hepática;    Fígado;    Estradiol;    Hepatectomia;   

DOI  :  10.1590/S0102-86502009000100002

来源: SciELO

【 摘 要 】

PURPOSE: To recognize the regenerative capacity influenced by the administrating of estradiol. METHODS: 42 female Wistar rats were used, divided into two groups, the control and the experiment group. A resection of approximately 70% of the liver was made in the liver of these animals. The control group received an intramuscular injection of one ml of peanut oil. The experiment group were given estradiol hexahydrobenzoate (50µg) diluted in one ml of peanut oil. Calibrations were done after 36 hours and 7 days, using three methods: the formula of Kwon et al.21, to recognize gain in volume, counting of the mitosis figures in five fields and the percentage of positive PCNA nuclei. RESULTS: Gain in volume (mass) was similar in both groups after 36 hours (p=0.1873) and higher in the experiment groups after seven days (p=0.0447). Microscopy showed a similar number of mitosis figures after 36 hours (p=0.3528) and a tendency to be higher in the experiment group after 7 days (p=0.0883). The average of positive PCNA nuclei was higher in the experiment group both after 36 hours (p=0.0009) and 7 days (p=0.0000). CONCLUSION: The estradiol hexahydrobenzoate improved liver regeneration in rats submitted to a 70% hepatectomy.

【 授权许可】

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