Effects of blood storage on ice in biochemical and arterial blood gas analysis of rats

Acta Cirurgica Brasileira

Marcus Vinicius Henriques Brito¹  Izabella Cristina Cristo Cunha¹  Mayra Gonçalves Aragón¹  Thiago Gabbay Martins Braga¹  Fabrício Diniz De Lima¹ 

[1] ,UEPA,Brazil

关键词: Blood Preservation;    Blood Gas Analysis;    Rats;    Preservação de Sangue;    Gasometria;    Ratos;   

DOI  :  10.1590/S0102-86502008000500013

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the effects of blood storage in biochemical and arterial blood gas analysis of Wistar rats. METHODS: Ten adult male rats with weights between 300-350 g were used. The catheterization of the internal carotid artery were performed, followed by withdrawal of 3 ml of blood using 3 separate syringes each containing 1 ml. The syringes were fulfilled one after another and numerated according to the order of collection. Then, the following groups were devised: GT1 - samples that were firstly analyzed in 20 minutes and the second exam 65 minutes after the collection; GT2 - samples that were firstly analyzed in 35 minutes and the second exam 80 minutes after the collection; GT3 - samples that were firstly analyzed in 50 minutes and the second exam 95 minutes after the collection. The samples were stored in a container with a temperature between 0 Cº and 4 Cº. Upon analysis the values of pH, PaCO2, PaO2, HCO3-, SatO2, SBE, Na+ and K+ concentrations and glucose were compared. RESULTS: No statistically relevant difference (p<0.05) between the GT1, GT2 or GT3, when measuring pH, PaCO2, PaO2, HCO3-, SatO2, SBE or glucose, were found. However, Na+ concentration decreased and the K+ concentration increased (p<0.05) when comparing the first analysis (20 minutes) with the last one (95 minutes). CONCLUSION: The blood storage on ice does not interfere in the results of arterial blood gas analysis for the period of at least 95 minutes, except for the Na+ e K+ levels, which are practicable until 80 minutes after storage.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License