Experimental model of severe acute pancreatitis in rabbits

Acta Cirurgica Brasileira

Alberto Goldenberg²  Ana Celia Diniz Cabral Barbosa Romeo¹  Márcia Bento Moreira¹  Franz Robert Apodaca²  Marcelo Moura Linhares²  Jacques Matone¹ 

[1] ,UNIFESP Division Gastrointestinal Surgery Department of SurgerySão Paulo,Brazil

关键词: Pancreatitis;    Acute Necrotizing;    Animal Experimentation;    Rabbits;    Pancreatite Necrosante Aguda;    Experimentação Animal;    Coelhos;   

DOI  :  10.1590/S0102-86502007000500008

来源: SciELO

【 摘 要 】

PURPOSE: To develop an experimental model of severe acute pancreatitis in rabbits through a pancreatic ductal injection of sodium taurocholate. METHODS: Twenty-four albino rabbits of the New Zealand lineage were distributed into four groups of six animals (A, B, C and S). The rabbits of three experimental groups (A, B and C) were submitted to a laparatomy and received a pancreatic ductal injection of 1ml/kg sodium taurocholate 5%. Also, they were submitted to further laparatomies after 4h, 8h and 12h, respectively. The control group (S) was subdivided into two groups of three animals: in subgroup S1 only the pancreatic duct catheterization was performed whereas in subgroup S2 the pancreatic duct catheterization as well as an injection of 1ml/kg physiologic solution 0.9% were carried out. After 12 hours, the rabbits were evaluated. In the re-intervention, blood was collected to determine the amylasemia and a pancreatectomy was carried out to investigate interstitial infiltration, steatonecrosis and necrosis of the organ, using an optical microscope. RESULTS: There was an elevation of amylase in all groups thus proving the existence of acute pancreatitis. The size of the interlobular septum increased progressively with a greater variation between group S1 (0.13) and group C (0. 53) (p=0.035). While all the animals in group A exhibited focal cellular necrosis, it was more intense in the rabbits of group B and culminated with a high proportion of severe pancreatic necrosis in group C animals. The difference in the intensity of cellular necrosis showed statistic significance (p=0.001). CONCLUSION: The proposed experimental model demonstrated its reproducibility and effectiveness in producing severe acute pancreatitis in rabbits.

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