Study of superoxide dismutase's expression in the colon produced by azoxymethane and inositol hexaphosfate's paper, in mice

Acta Cirurgica Brasileira

Eva Glória Abrão Siufi Do Amaral²  Djalma José Fagundes¹  Guido Marks²  Celso Massachi Inouye² 

[1] ,Federal University of Mato Grosso do Sul Department of Surgery ,Brazil

关键词: Colon;    Superoxide Dismutase;    Phytic Acid;    Carcinogens;    Azoxymethane;    Rats;    Colo;    Superóxido Dismutase;    Àcido Fítico;    Carcinógeno;    Azoximetano;    Ratos;   

DOI  :  10.1590/S0102-86502006001000007

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the expression of superoxide dismutase (SOD), with use of antioxidant inositol hexaphosfate, in the presence of the carcinogen azoxymethane, in FCA of colon rats. METHODS: Wistar rats (n=48) were distributed in four groups of 12 mice. Divided in control (n=12); with azoxymethane administration AOM (n=12); administration of IP6 (n=12) and with administration of IP6/AOM (n=12). The subcutaneous administration of azoxymethane happened in the week 3 and 4 of the experiment, in dose 20mg/Kg, weekly; and administration of IP6 to 1% in water of drinking for 6 weeks in the group 3 and 4. The identification of the expression SOD-1 was accomplished through the quantification imunohistochemistry by the image processing attended by computer in crypts and focus of aberrant crypts in right colon. RESULTS: The group control presented expression of SOD1, on average 16,0%; group AOM, 26,7%; group IP6, 16,9%; group IP6/AOM, 20,9%. Variance analysis among the groups, was calculated 0,0078. CONCLUSION: The azoxymethane increase expression SOD1, while inositol hexaphosphate decreases in a significant way the expression of SOD1 promoted by the administration of the carcinogen azoxymethane.

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