Effects of simvastatin in abdominal sepsis in rats

Acta Cirurgica Brasileira

José Luiz De Souza Neto²  Irami Araújo Filho²  Amália Cínthia Meneses Do Rego¹  Víctor Almeida Dominici¹  Ítalo Medeiros Azevedo¹  Eryvaldo Sócrates Tabosa Egito¹  José Brandão-neto¹  Aldo Cunha Medeiros¹ 

[1] ,UFRN Program in Health Sciences ,Brazil

关键词: Statin;    Inflammation;    Abdominal sepsis;    Wistar rat;    Cytokine;    Leukocyte;    Estatina;    Inflamação;    Sepse abdominal;    Rato Wistar;    Citocinas;    Leucócitos;   

DOI  :  10.1590/S0102-86502006001000003

来源: SciELO

【 摘 要 】

PURPOSE: Statins are widely recognized as hypolipemic drugs, but some studies have observed anti-inflammatory and immunomodulatory effects, known as pleiotropic. The aims of this work was to study possible anti-inflammatory effects of simvastatin in abdominal sepsis. Serum pro-inflammatory cytokines and leukocytes count were determined in an experimental model of abdominal sepsis, using cecal ligation and puncture (CLP) in rats. METHODS: Twenty eigth Wistar rats weighing 285±12g were randomly divided in: CLP/Sinvastatin rats (n=7), treated with 10 mg/Kg of oral simvastatin 18 and 2 hs berofe CLP; CLP/Saline group rats (n=7), treated with oral saline; group Sham/Simvastatin (n=7), treated with simvastatin, and group Sham/Saline (n=7), treated with saline. Serum TNF-alpha, IL-1beta and IL-6 by ELISA and total leukocytes, neutrophils, lymphocytes, and eosinophils were determined 24 hs after CLP. ANOVA and Tukey test were used considering significant p<0.05. RESULTS: It was demonstrated that serum TNF-alpha, IL-1beta and IL-6 were respectively 364,8±42pg/mL; 46,3±18pg/mL and 28,4±13pg/mL in CLP/Sinvastatin rats, significantly lower (p<0.05) than in group CLP/Saline (778,5±86pg/ml; 176,9±46pg/ml; 133,6±21 pg/ml, respectively). The same results were observed in total leukocytes and neutrophils counts. CONCLUSION: These results clearly demonstrate that simvastatin is an effective agent that reduces cytokines levels and leukocyte count in sepsis, independently of its well-known lipid-lowering effects. Thus, HMG-CoA reductase inhibitors like simvastatin have important anti-inflammatory effects in abdominal sepsis in rats.

【 授权许可】

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