Is subdiaphragmatic aortic cross-clamping a suitable model for spinal cord ischemia/reperfusion injury study in rats?

Acta Cirurgica Brasileira

Sonia Elizabeth Lopez Carrillo¹  Sérgio Botelho Guimarães²  Paulo Roberto Cavalcante De Vasconcelos¹  Paulo Roberto Leitão De Vasconcelos¹ 

[1] ,UFC Department of Surgery ,Brazil

关键词: Ischemia;    Reperfusion Injury;    Spinal Cord;    Models;    Animal;    Rats;    Isquemia;    Traumatismo por Reperfusão;    Medula Espinhal;    Modelos Animais;    Ratos;   

DOI  :  10.1590/S0102-86502006000400006

来源: SciELO

【 摘 要 】

PURPOSE: To evaluate the efficacy of subdiaphragmatic aortic cross-clamping in an experimental model of ischemia/reperfusion injury of the spinal cord in albino rats. METHODS: Thirty-six male Wistar rats were randomized in two groups (n=18): G-1 (Sham) and G-2 (Ischemia/Reperfusion, I/R). G-2 rats were submitted to 30 min subdiafragmatic aortic cross-clamping. G-1 rats served as controls and were submitted to surgical trauma (laparotomy) without ischemia. Samples (spinal cord and arterial blood) were collected at the end of ischemic period and 10 (T-10) and 20 (T-20) min later in G-2 rats. Sham rats (G-1) samples were collected at the same time-points. Blood and tissue metabolites concentrations of pyruvate, lactate, glucose and medullary adenosine triphosphate (ATP) were assayed. RESULTS: Blood and tissue concentrations of pyruvate and glucose as well as lactate and medullary ATP were not different when comparing G1 to G2. Lactacemia was significantly elevated in G-2 compared with G-1 rats during reperfusion (T-10). CONCLUSION: Subdiaphragmatic aortic cord cross-clamping is not a suitable rat model for spinal cord ischemia/reperfusion injury study as it does not ensure changes in in vivo tissue metabolites concentrations similar to those found in tissues subjected to ischemia/reperfusion.

【 授权许可】

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