Ischemic preconditioning and the gene expression of enteric endothelial cell biology of rats submitted to intestinal ischemia and reperfusion

Acta Cirurgica Brasileira

Murched Omar Taha²  Regiane Miranda Ferreira¹  Nabiha Saadi Abrahão Taha¹  Hugo Pequeno Monteiro²  Afonso Caricati-neto²  Itamar Souza Oliveira-júnior²  Djalma José Fagundes² 

[1] ,UNIFESPSao Paulo SP ,Brazil

关键词: Ischemia;    Reperfusion Injury;    Endothelial Cells;    Gene Expression Profiling;    Rats;   

DOI  :  10.1590/S0102-86502013000300002

来源: SciELO

【 摘 要 】

PURPOSE: To investigate the effects of ischemic preconditioning (IPC) on the expression of pro and anti-apoptotic genes in rat endothelial cells undergoing enteric ischemia (I) and reperfusion (R). METHODS: Thirty rats underwent clamping of the superior mesenteric vessels. Sham group (GS) laparotomy only; Ischemia (GI): intestinal ischemia (60 min); Ischemia and Reperfusion (GIR): ischemia (60 min) and reperfusion (120 min); Ischemia and intestinal ischemic preconditioning (GI + IPC) : 5 minutes of ischemia followed by 10 min of reperfusion before sustained ischemia (60 min) ischemia and reperfusion and IPC (GIR + IPC): 5 min ischemia followed by 10 min of reperfusion before sustained ischemia (60min) and reperfusion (120 min). Rat Endothelial Cell Biology (PCR array) to determine the expression of genes related to endothelial cell biology. RESULTS: Gene expression of pro-apoptotic markers (Casp1, Casp6, Cflar, Fas, and Pgl) was down regulated in GI+IPC and in GIR + IPC. In contrast, the expression of anti-apoptotic genes (Bcl2 and Naip2), was up-regulated in GI + IPC and in GIR + IPC. CONCLUSION: Ischemic preconditioning may protect against cell death caused by ischemia and reperfusion.

【 授权许可】

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