Experimental model for establishment of hypoxia in 75 cm² culture flasks

Acta Cirurgica Brasileira

Sidney Mamoru Keira²  Lydia Masako Ferreira²  Alfredo Gragnani²  Ivone Da Silva Duarte²  Anelisa Bittencourt Campaner¹  Marcelino De Souza Durão Jr¹ 

[1] ,Federal University of São Paulo São Paulo Medical School Plastic Surgery Division

关键词: Cells;    cultured;    Hypoxia;    Cultura de células;    Hipóxia;   

DOI  :  10.1590/S0102-86502004000700006

来源: SciELO

【 摘 要 】

In Plastic Surgery, cell culture represents the perspective of studying cellular mechanisms that guide the healing process of several tissues. Some steps of the healing process depend on physical factors as the tissular partial pressure of O2. In cell culture, it is possible to submit cells to hypoxic enviroment. The present study reports an alternative method at low cost for the establishment of hypoxic environment in cell culture flasks.

【 授权许可】

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