Experimental model for collagen estimation in cell culture

Acta Cirurgica Brasileira

Sidney Mamoru Keira²  Lydia Masako Ferreira²  Alfredo Gragnani²  Ivone Da Silva Duarte²  José Barbosa¹ 

[1] ,Federal University of São Paulo São Paulo Medical School Plastic Surgery Division

关键词: Cells;    cultured;    Collagen;    Dye;    Cultura de células;    Colágeno;    Corante;   

DOI  :  10.1590/S0102-86502004000700005

来源: SciELO

【 摘 要 】

In the study of Plastic Surgery, cell culture may be used at experimental level in researches concerning biosynthesis functions of skin cells such as fibroblasts, keratinocytes, adipocytes, chondrocytes and osteocytes. The present study reports an experimental model for estimation of collagen in cell cultures using chromogenic precipitation reaction with an especific dye (Sirius Red).

【 授权许可】

CC BY   
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