Experimental model of cultured keratinocytes

Acta Cirurgica Brasileira

Alfredo Gragnani²  Jeffrey R. Morgan¹  Lydia Masako Ferreira¹ 

[1] ,UNIFESP EPM

关键词: Keratinocyte;    Human acellular dermis;    Skin transplantation;    Fibroblasts;    Cultured cell;    Queratinócitos;    Derme acelular humana;    Transplante de pele;    Fibroblastos;    Cultura de células;   

DOI  :  10.1590/S0102-86502003001100002

来源: SciELO

【 摘 要 】

The bioengineering research is essential in the development of ideal combination of biomaterials and cultured cells to produce the permanent wound coverage. The experimental model of cultured keratinocytes presents all steps of the culture, since the isolation of the keratinocytes, preparation of the human acellular dermis, preparation of the composite skin graft and their elevation to the air-liquid interface. The research in cultured keratinocytes model advances in two main ways: 1. optimization of the methods in vitro to the skin cells culture and proliferation and 2. developing biomaterials that present similar skin properties.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License