Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis

Revista Brasileira de Cirurgia Cardiovascular

Sydney Correia Leão¹  Michael R. Dashwood¹  Mateus Santana De Andrade¹  Nicolas Nascimento Santos¹  Olivia Regina Lins Leal Teles¹  Williasmin Batista De Souza¹  Tania Maria De Andrade Rodrigues¹ 

关键词: Rheumatic Fever;    Mitral Valve Stenosis;    Endothelin- 1;    Immunohistochemistry;    Blotting;    Western;    Febre Reumática;    Estenose da Valva Mitral;    Endotelina-1;    Imunoistoquímica;    Western Blotting;   

DOI  :  10.5935/1678-9741.20150004

来源: SciELO

【 摘 要 】

AbstractIntroduction:Rheumatic Fever represents a serious public health problem in developing countries, with thousands of new cases each year. It is an autoimmune disease, which occurs in response to infection by streptococcus A.Objective:The aim of this study was to evaluate the immunolabeling and protein expression for endothelin-1 and 3 (ET-1, ET-3) and its receptors (ETA, ETB) in rheumatic mitral valves.Methods:Immunohistochemistry was used to identify ET-1/ET-3 and ETA/ETB receptors in rheumatic and control mitral valves. Quantitative analysis of immunostaining for ET-1/ET-3 and ETA/ETB receptors was performed. In addition, western blot analysis was carried out to assess protein levels in tissue samples.Results:ET-1 and ETA receptor immunostaining predominated in stenotic valves, mainly associated with fibrotic regions, inflammatory areas and neovascularization. Quantitative analysis showed that the average area with positive expression of ET-1 was 18.21±14.96%. For ETA and ETB, the mean expressed areas were respectively 15.06±13.13% and 9.20±11.09%. ET-3 did not have a significant expression. The correlation between the expression of both endothelin receptors were strongly positive (R=0.74, P=0.02), but the correlation between ET-1 and its receptor were negative for both ETA (R=-0.37, P=0.25), and ETB (R=-0.14, P=0.39). This data was supported by western blot analysis.Conclusion:The strong correlation between ET-1 and its receptors suggests that both play a role in the pathophysiology of rheumatic mitral valve stenosis and may potentially act as biomarkers of this disease.

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