期刊论文详细信息
Revista Brasileira de Cirurgia Cardiovascular
Analysis of immunostaining and western blotting of endothelin 1 and its receptors in mitral stenosis
Sydney Correia Leão1  Michael R. Dashwood1  Mateus Santana De Andrade1  Nicolas Nascimento Santos1  Olivia Regina Lins Leal Teles1  Williasmin Batista De Souza1  Tania Maria De Andrade Rodrigues1 
关键词: Rheumatic Fever;    Mitral Valve Stenosis;    Endothelin- 1;    Immunohistochemistry;    Blotting;    Western;    Febre Reumática;    Estenose da Valva Mitral;    Endotelina-1;    Imunoistoquímica;    Western Blotting;   
DOI  :  10.5935/1678-9741.20150004
来源: SciELO
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【 摘 要 】

AbstractIntroduction:Rheumatic Fever represents a serious public health problem in developing countries, with thousands of new cases each year. It is an autoimmune disease, which occurs in response to infection by streptococcus A.Objective:The aim of this study was to evaluate the immunolabeling and protein expression for endothelin-1 and 3 (ET-1, ET-3) and its receptors (ETA, ETB) in rheumatic mitral valves.Methods:Immunohistochemistry was used to identify ET-1/ET-3 and ETA/ETB receptors in rheumatic and control mitral valves. Quantitative analysis of immunostaining for ET-1/ET-3 and ETA/ETB receptors was performed. In addition, western blot analysis was carried out to assess protein levels in tissue samples.Results:ET-1 and ETA receptor immunostaining predominated in stenotic valves, mainly associated with fibrotic regions, inflammatory areas and neovascularization. Quantitative analysis showed that the average area with positive expression of ET-1 was 18.21±14.96%. For ETA and ETB, the mean expressed areas were respectively 15.06±13.13% and 9.20±11.09%. ET-3 did not have a significant expression. The correlation between the expression of both endothelin receptors were strongly positive (R=0.74, P=0.02), but the correlation between ET-1 and its receptor were negative for both ETA (R=-0.37, P=0.25), and ETB (R=-0.14, P=0.39). This data was supported by western blot analysis.Conclusion:The strong correlation between ET-1 and its receptors suggests that both play a role in the pathophysiology of rheumatic mitral valve stenosis and may potentially act as biomarkers of this disease.

【 授权许可】

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