Sanguineous normothermic, intermittent cardioplegia, effects on hypertrophic myocardium: morphometric, metabolic and ultrastructural studies in rabbits hearts

Revista Brasileira de Cirurgia Cardiovascular

Clovis Carbone Junior²  José Eduardo De Salles Roselino¹  Valder Rodrigues Mello²  Paulo Roberto Barbosa Evora¹  Albert Amin Sader² 

[1] ,University of São Paulo Ribeirão Preto Faculty of Medicine Department of Surgery and AnatomyRibeirão Preto SP ,Brazil

关键词: Heart arrest;    induced;    Hypertrophy;    left ventricular;    Cardiovascular surgical procedures;    Parada cardíaca induzida;    Hipertrofia ventricular esquerda;    Procedimentos cirúrgicos cardiovasculares;   

DOI  :  10.5935/1678-9741.20120105

来源: SciELO

【 摘 要 】

OBJECTIVES: The present investigation aimed to study the protective effect of intermittent normothermic cardioplegia in rabbit's hypertrophic hearts. METHODS: The parameters chosen were 1) the ratio heart weight / body weight, 2) the myocardial glycogen levels, 3) ultrastructural changes of light and electron microscopy, and 4) mitochondrial respiration. RESULTS: 1) The experimental model, coarctation of the aorta induced left ventricular hypertrophy; 2) the temporal evolution of the glycogen levels in hypertrophic myocardium demonstrates that there is a significant decrease; 3) It was observed a time-dependent trend of higher oxygen consumption values in the hypertrophic group; 4) there was a significant time-dependent decrease in the respiratory coefficient rate in the hypertrophic group; 5) the stoichiometries values of the ADP: O2 revealed the downward trend of the values of the hypertrophic group; 6) It was possible to observe damaged mitochondria from hypertrophic myocardium emphasizing the large heterogeneity of data. CONCLUSION: The acquisition of biochemical data, especially the increase in speed of glycogen breakdown, when anatomical changes are not detected, represents an important result even when considering all the difficulties inherent in the process of translating experimental results into clinical practice. With regard to the adopted methods, it is clear that morphometric methods are less specific. Otherwise, the biochemical data allow detecting alterations of glycogen concentrations and mitochondria respiration before the morphometric alterations should be detected

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