Sulfated polysaccharide extracted of the green algae Caulerpa racemosa increase the enzymatic activity and paw edema induced by sPLA2 from Crotalus durissus terrificus venom

Revista Brasileira de Farmacognosia

Camila L. Pires²  Selma D. Rodrigues²  Daniel Bristot²  Henrique Hessel Gaeta²  Daniela De Oliveira Toyama¹  Wladimir Ronald Lobo Farias¹  Marcos Hikari Toyama² 

[1] ,Universidade Estadual Paulista Laboratório de Biologia Molecular e Peptídeos ,Brazil

关键词: Crotalus durissus terrificus;    edematogenic effect;    myotoxic activity;    seaweed;    secretory;    phospholipase A2;    sulfated polysaccharides;   

DOI  :  10.1590/S0102-695X2013005000050

来源: SciELO

【 摘 要 】

Sulfated polysaccharides derived from seaweed have shown great potential for use in the development of new drugs. In this study, we observed that a low-molecular-weight sulfated polysaccharide from Caulerpa racemosa, termed CrSP, could interact with secretory phospholipase A2 (sPLA2) isolated from Crotalus durissus terrificus venom. When native sPLA2 (14 kDa) was incubated with CrSP, they formed a molecular complex (sPLA2:CrSP) with a molecular mass of 32 kDa, approximately. Size exclusion chromatography experiments suggested that CrSP formed a stable complex with sPLA2. We belived that sPLA2 and SPCr are involved an ionic interaction between negatively charged CrSP and the positively charged basic amino acid residues of sPLA2, because this interaction induced significant changes in sPLA2 enzymatic and pharmacological activities. CrSP caused a significant increase in sPLA2 enzymatic and bactericidal activity and increased its edematogenic effect. A pharmacological assay showed that the myotoxic activity of sPLA2:CrSP is unrelated to its enzymatic activity and that sPLA2:CrSP may have a practical application as a natural antibacterial agent for use in humans and commercially raised animals.

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