Antifungal activity of raw extract and flavanons isolated from Piper ecuadorense from Ecuador

Revista Brasileira de Farmacognosia

Jorge Ramirez²  Luis Cartuche²  Vladimir Morocho²  Silvio Aguilar¹  Omar Malagon² 

[1] ,Universidad Tecnica Particular de Loja Seccion Quimica Basica y Aplicada Departamento de Quimica,Ecuador

关键词: antifungal activity;    flavonoids;    pinocembrin;    pinostrobin;    Piper ecuadorense;    matico de monte;   

DOI  :  10.1590/S0102-695X2013005000012

来源: SciELO

【 摘 要 】

The MeOH extract of Piper ecuadorense Sodiro, Piperaceae, was chosen for metabolite isolation and elucidation due to the strong antifungal activity exhibited, measured by means of the broth microdilution method. Two known flavonoids: pinostrobin (1) and pinocembrin (2) were isolated from 4.16 g. of dichloromethane extract by column chromatography, using a gradient of hexane/EtOAc. A total of 20 mg of 1 were obtained from the fraction eluted with hexane-EtOAc 95:5 v/v, and 100 mg of 2 were obtained from the fraction eluted with hexane-EtOAc 85:15 v/v. The MIC values of the MeOH extract was 31.25 µg/mL for Trichophyton mentagrophytes ATCC® 28185 and 62.5 µg/mL for Trichophyton rubrum ATCC® 28188. The MIC value of pinocembrin was 125 µg/mL for Trichophyton mentagrophytes ATCC® 28185 and Trichophyton rubrum ATCC® 28188. Pinostrobin in antifungal test was not active against fungi tested.

【 授权许可】

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