Immunomodulatory and antibacterial activities of extracts from Rutaceae species

Revista Brasileira de Farmacognosia

José Fernando Oliveira Costa²  Paulo Juiz²  André São Pedro²  Juceni P. De L. David¹  Jorge M. David¹  Ana Maria Giulietti¹  Flávio França¹  Ricardo Ribeiro Dos Santos²  Milena B. P. Soares² 

[1] ,Fundação Oswaldo Cruz Centro de Pesquisas Gonçalo Moniz Laboratório de Engenharia Tecidual e ImunofarmacologiaSalvador BA ,Brazil

关键词: Immunomodulatory activity;    Rutaceae;    antibacterial activity;    Atividade imunomoduladora;    Rutaceae;    atividade antibacteriana;   

DOI  :  10.1590/S0102-695X2010000400007

来源: SciELO

【 摘 要 】

Rutaceae is a taxon with species very well distributed in Brazilian semi-arid area, commonly used in folk medicine. Species from this genus have diverse biological activity described in literature. In this work, immunomodulatory and bactericidal activity are described for chloroform and ethyl acetate extracts of three of them (Esenbeckia grandiflora Mart., Pilocarpus spicatus A.St.-Hil. and Galipea simplicifolia Schult.). Initially all the samples had their cytotoxicity evaluated, aiming to determine the LC50. The immunomodulatory potential was evaluated in cultures of murine splenocytes stimulated or not with concanavalin A and in a mixed lymphocyte reaction (MLR) using splenocytes from BALB/c (H-2d) mice immunized with splenocytes from C57Bl/6 (H-2b) mice. Four samples had higher values of lymphoproliferation inhibition in concanavalin A-stimulated cultures and were evaluated in MLR. The antibacterial activity of extracts was also evaluated and the minimal inhibitory concentrations (MIC) for two active samples were 1.0 and 5.0 mg/ml for extracts from Esenbeckia grandiflora Mart. and Galipea simplicifolia Schult., respectively. Thus, our results reinforce data of literature relating biological activity for many species of the Rutaceae family and encourage studies with these species aiming to discover active compounds, candidates to new medicines.

【 授权许可】

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