In vitro morphogenesis and cell suspension culture establishment in Piper solmsianum C. DC. (Piperaceae)

Acta Botanica Brasilica

Tiago Santana Balbuena²  Claudete Santa-catarina²  Vanildo Silveira¹  Massuo Jorge Kato¹  Eny Iochevet Segal Floh² 

[1] ,Universidade de São Paulo Instituto de Biociências Departamento de BotânicaSão Paulo SP ,Brasil

关键词: callogenesis;    cell suspension culture;    morphogenesis;    pariparoba;    plant growth regulators;    calogênese;    morfogênese;    pariparoba;    reguladores de crescimento;    suspensões celulares;   

DOI  :  10.1590/S0102-33062009000100029

来源: SciELO

【 摘 要 】

Piper solmsianum is a shrub from Southeast Brazil in which many biologically active compounds were identified. The aim of this work was to establish a cell suspension culture system for this species. With this in mind, petiole and leaf explants obtained from in vitro plantlets were cultured in the presence of different plant growth regulator combinations (IAA, NAA, 2,4-D and BA). Root and indirect shoot adventitious formation, detected by histological analysis, was observed. Besides the different combinations of plant growth regulators, light regime and the supplement of activated charcoal (1.5 mg.l-1) were tested for callus induction and growth. Cultures maintained in light, on a 0.2 mg.l-1 2,4-D and 2 mg.l-1 BA supplemented medium, and in the absence of activated charcoal, showed the highest calli fresh matter increment. From a callus culture, cell suspension cultures were established and their growth and metabolite accumulation studied. The achieved results may be useful for further characterization of the activated secondary metabolites pathways in in vitro systems of P. solmsianum.

【 授权许可】

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