Antileishmanial activity of hydroalcoholic extract and fractions obtained from leaves of Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.)

Revista Brasileira de Farmacognosia

Dias Filho, Benedito P.¹  Luize, Patrícia S.¹  Nakamura, Celso V.¹  Vendrametto, Michele C.¹  Cortez, Diógenes A.G.¹  Universidade Estadual de Maringá, Maringá, Brasil¹  Ueda-Nakamura, Tânia¹  Santos, Adriana O.¹ 

关键词: Piper regnellii;    Leishmania amazonensis;    pariparoba;    antileishmanial activity;    promastigote.;   

DOI  :  10.1590/S0102-695X2006000100011

来源: Sociedade Brasileira de Farmacognosia

【 摘 要 】

Biological activity of the crude extract and several fractions obtained from Piper regnellii var. pallescens was assessed on Leishmania amazonensis. This study included the extraction process and bioassay-guided fractionation by the adsorption chromatography method. A progressive increase in the antileishmanial effect was observed in the course of the purification process. The hydroalcoholic extract water soluble (EBA) had a 50% inhibitory concentration (IC50) at 167 µg/mL whereas the hydroalcoholic extract acetate soluble (EBAcOEt) showed an IC50 of 30 µg/mL against the growth of promastigote forms after 48 h of culturing. The hexan fraction (FHex) showed an antileishmanial activity greater than EBAcOEt with IC50 at 21.5 µg/mL. Analysis of cytotoxicity indicated that the toxic concentrations of the EBA, EBAcOEt, and fractions were higher for J774G8 macrophages than for the protozoans.

【 授权许可】

Unknown