Revista Brasileira de Farmacognosia | |
Antileishmanial activity of hydroalcoholic extract and fractions obtained from leaves of Piper regnellii (Miq.) C. DC. var. pallescens (C. DC.) | |
Dias Filho, Benedito P.1  Luize, Patrícia S.1  Nakamura, Celso V.1  Vendrametto, Michele C.1  Cortez, Diógenes A.G.1  Universidade Estadual de Maringá, Maringá, Brasil1  Ueda-Nakamura, Tânia1  Santos, Adriana O.1  | |
关键词: Piper regnellii; Leishmania amazonensis; pariparoba; antileishmanial activity; promastigote.; | |
DOI : 10.1590/S0102-695X2006000100011 | |
来源: Sociedade Brasileira de Farmacognosia | |
【 摘 要 】
Biological activity of the crude extract and several fractions obtained from Piper regnellii var. pallescens was assessed on Leishmania amazonensis. This study included the extraction process and bioassay-guided fractionation by the adsorption chromatography method. A progressive increase in the antileishmanial effect was observed in the course of the purification process. The hydroalcoholic extract water soluble (EBA) had a 50% inhibitory concentration (IC50) at 167 µg/mL whereas the hydroalcoholic extract acetate soluble (EBAcOEt) showed an IC50 of 30 µg/mL against the growth of promastigote forms after 48 h of culturing. The hexan fraction (FHex) showed an antileishmanial activity greater than EBAcOEt with IC50 at 21.5 µg/mL. Analysis of cytotoxicity indicated that the toxic concentrations of the EBA, EBAcOEt, and fractions were higher for J774G8 macrophages than for the protozoans.
【 授权许可】
Unknown
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