Effects of sucrose and irradiance on germination and early gametophyte growth of the endangered tree fern Dicksonia sellowiana Hook (Dicksoniaceae)

Acta Botanica Brasilica

Gladys Daniela Rogge Renner¹  Áurea Maria Randi¹ 

[1] ,University Federal of Santa Catarina Department of Botany Laboratory of Plant PhysiologyFlorianópolis SC ,Brazil

关键词: sugar;    chlorophyll;    gametophyte;    germination;    irradiance;    açúcar;    clorofila;    gametófito;    germinação;    irradiância;   

DOI  :  10.1590/S0102-33062004000200017

来源: SciELO

【 摘 要 】

It is an endangered tree fern that grows in mixed umbrophylus forests in the tropics. Sterilized spores were germinated at 25 ± 2ºC under a 16-hour photoperiod, in Dyer and MS medium supplemented with 0 to 5% sucrose. The germination was lower with the addition of sucrose. Dry mass was higher in 30-day-old gametophytes cultivated in Dyer medium with the addition of 3 to 5% of sucrose. The dry mass decreased in 30-day-old gametophytes cultivated in Dyer and MS media without sucrose and in MS medium with the addition of 4 or 5% of sucrose. The effect of different irradiance on the germination and early gametophyte development of D. sellowiana was analyzed in the autumn of 1998 (May until July). Conical flasks containing spores were kept over a period of 49 days in 50cm³ boxes covered with black shade netting, which gave 5, 20, 36 and 50% of irradiance. The lower mean germination time was observed for spores cultivated under 5% and 20% of irradiance. The highest chlorophyll content was recorded in gametophytes cultivated for 49 days under 20% and 5% of irradiance .The highest soluble sugar content was recorded in gametophytes cultivated under 5% and 20% of irradiance.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License