Extraction optimization of antioxidant polysaccharides from leaves of Gynura bicolor (Roxb. & Willd.) DC

Food Science and Technology (Campinas)

Fen Yan¹  Xuena Yang¹  Chang Liu¹  Shangrong Huang¹  Lan Liao¹  Caili Fu¹ 

[1] ,Fuzhou University College of Bioscience & Biotechnology Fuzhou PR ,China

关键词: Gynura bicolor;    polysaccharide;    orthogonal design;    antioxidant activity;   

DOI  :  10.1590/fst.2014.0055

来源: SciELO

【 摘 要 】

Orthogonal design was employed to study the effect of extraction time, temperature and liquid-to-solid ratio on the production of antioxidant polysaccharides from leaves of Gynura bicolor (PLG). Analysis of variance was performed on the data obtained. The most relevant variable was extraction time. A liquid-solid ratio of 30:1 (v/w), a temperature of 80 °C and an extraction time of 3 h were found to be optimal for PLG. The optimal extraction yield of 4.9% was obtained through additional verification test. Hydroxyl radical-scavenging activity, reducing power and ferrous ion chelating ability of PLG were determined. PLG possess concentration-dependent antioxidant potency and IC50 of PLG was 4.67, 0.24 and 4.31 mg/mL for hydroxyl radical-scavenging and ferric ion chelating abilities as well as reducing power, respectively. The results suggest that G. bicolor polysaccharides could be potential source of natural antioxidant and be contributor to the health benefits of G. bicolor.

【 授权许可】

CC BY   
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