Spirulina platensis: process optimization to obtain biomass

Food Science and Technology (Campinas)

Iracema De Oliveira Moraes²  Regina De Oliveira Moraes Arruda²  Natália Rocha Maresca¹  Aline De Oliveira Antunes¹  Rodrigo De Oliveira Moraes² 

[1] ,PROBIOM TECNOLOGIA Pesquisa e Desenvolvimento Experimental em Ciências Físicas e Naturais LtdaCampinas SP ,Brasil

关键词: Spirulina platensis;    human food;    biomass;    cyanobacteria;    Spirulina platensis;    alimento;    biomassa;    cianobactéria;   

DOI  :  10.1590/S0101-20612013000500026

来源: SciELO

【 摘 要 】

Spirulina platensis is a photoautotrophic mesophilic cyanobacterium. Its main sources of nutrients are nitrate, urea, and ammonium salts. Spirulina cultivation requires temperature, light intensity, and nutrient content control. This microalgae has been studied and used commercially due to its therapeutic and antioxidant potential. In addition, several studies have reported its ability to use CO2, its immune activity, and use as an adjuvant nutritive factor in the treatment of obesity. The objective of this study is the production of biomass of S. platensis using different rates of stirring, nitrogen source, amount of micronutrients, and luminosity. A 2(4) experimental design with the following factors: stirring (120 and 140 RPM), amount of nitrogen (1.5 and 2.5 g/L), amount of micronutrients (0,25 and 0,75 mL/L) (11 and 15 W), and luminosity was used. Fermentation was performed in a 500 mL conical flask with 250 mL of culture medium and 10% inoculum in an incubator with controlled stirring and luminosity. Fermentation was monitored using a spectrophotometer (560 nm), and each fermentation lasted 15 days. Of the parameters studied, luminosity is the one with the highest significance, followed by the amount of nitrogen and the interaction between stirring and micronutrients. Maximum production of biomass for 15 days was 2.70 g/L under the following conditions: luminosity15W; stirring, 120 RPM; source of nitrogen, 1.5 g/L; and micronutrients, 0.75 mL/L.

【 授权许可】

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