Short-term levosimendan treatment protects rat testes against oxidative stress

Brazilian Journal of Medical and Biological Research

M.b. Yuksel²  S. Kavak¹  I. Gecit¹  H. Basel¹  H.a. Gümrükçüoğlu¹  H. Demir¹  İ. Meral¹ 

[1] ,The State Hospital of Mus Urology Clinic Mus,Turkey

关键词: Levosimendan;    Oxidative stress;    Reactive oxygen species;    Rat testes;    SOD;    GSH-Px;    MDA;   

DOI  :  

来源: SciELO

【 摘 要 】

The objective of this study was to evaluate the effect of short-term levosimendan exposure on oxidant/antioxidant status and trace element levels in the testes of rats under physiological conditions. Twenty male Wistar albino rats were randomly divided into two groups of 10 animals each. Group 1 was not exposed to levosimendan and served as control. Levosimendan (12 µg/kg) diluted in 10 mL 0.9% NaCl was administered intraperitoneally to group 2. Animals of both groups were sacrificed after 3 days and their testes were harvested for the determination of changes in tissue oxidant/antioxidant status and trace element levels. Tissue malondialdehyde (MDA) was significantly lower in the levosimendan group (P < 0.001) than in the untreated control group and superoxide dismutase and glutathione peroxidase (GSH-Px) levels were significantly higher in the levosimendan group (P < 0.001). Carbonic anhydrase, catalase and GSH levels were not significantly different from controls. Mg and Zn levels of testes were significantly higher (P < 0.001) and Co, Pb, Cd, Mn, and Cu were significantly lower (P < 0.001) in group 2 compared to group 1. Fe levels were similar for the two groups (P = 0.94). These results suggest that 3-day exposure to levosimendan induced a significant decrease in tissue MDA level, which is a lipid peroxidation product and an indicator of oxidative stress, and a significant increase in the activity of an important number of the enzymes that protect against oxidative stress in rat testes.

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