Genotyping of methicillin-resistant Staphylococcus aureus isolates obtained in the Northeast region of Brazil

Brazilian Journal of Medical and Biological Research

F.c. De Sousa-junior²  M.c. Silva-carvalho¹  M.j.b.c. Fernandes²  M.f.p. Vieira¹  F.l.p.c. Pellegrino¹  A.m.s. Figueiredo¹  M.c.n. De Melo²  E.p. Milan¹ 

[1] ,Universidade Federal do Rio Grande do Norte Departamento de Microbiologia e Parasitologia

关键词: Methicillin-resistant Staphylococcus aureus;    MRSA;    Antimicrobial resistance;    Brazilian epidemic clone;    Pediatric clone;    SCCmecIV;   

DOI  :  10.1590/S0100-879X2009005000018

来源: SciELO

【 摘 要 】

Methicillin-resistant Staphylococcus aureus (MRSA) is a major agent of hospital infections worldwide. In Brazil, a multiresistant MRSA lineage (ST239-SCCmecIIIA), the so-called Brazilian epidemic clone (BEC), has predominated in all regions. However, an increase in nosocomial infections caused by non-multiresistant MRSA clones has recently been observed. In the present study, 45 clinical isolates of MRSA obtained from a university hospital located in Natal city, Brazil, were identified by standard laboratory methods and molecularly characterized using staphylococcal chromosome cassette mec (SCCmec) typing and pulsed-field gel electrophoresis. Antimicrobial susceptibility testing was carried out using CLSI methods. The MRSA isolates studied displayed a total of 8 different pulsed-field gel electrophoresis patterns (types A to H) with predominance (73%) of pattern A (BEC-related). However, MRSA harboring SCCmec type IV were also identified, 3 (7%) of which were genetically related to the pediatric clone - USA800 (ST5-SCCmecIV). In addition, we found a considerable genetic diversity within BEC isolates. MRSA displaying SCCmecIV are frequently susceptible to the majority of non-β-lactam antibiotics. However, emergence of multiresistant variants of USA800 was detected.

【 授权许可】

CC BY   
 All the contents of this journal, except where otherwise noted, is licensed under a Creative Commons Attribution License