An extract of Polygonum multiflorum protects against free radical damage induced by ultraviolet B irradiation of the skin

Brazilian Journal of Medical and Biological Research

I.k. Hwang²  K.-y. Yoo¹  D.w. Kim²  S.j. Jeong²  C.-k. Won¹  W.-k. Moon²  Y.s. Kim¹  D.y. Kwon¹  M.h. Won¹  D.-w. Kim² 

[1] ,Natural F&P Co. Ltd. Central Research Center Chunchon,South Korea

关键词: Polygonum multiflorum extract;    Skin;    Ultraviolet B irradiation;    Free radicals;    Cu;    Zn-superoxide dismutase;    Hairless mouse;   

DOI  :  10.1590/S0100-879X2006000900005

来源: SciELO

【 摘 要 】

Over the last decades, the incidence of ultraviolet B (UVB)-related skin problems has been increasing. Damages induced by UVB radiation are related to mutations that occur as a result of direct DNA damage and/or the production of reactive oxygen species. We investigated the anti-oxidant effects of a Polygonum multiflorum thumb extract against skin damage induced by UVB irradiation. Female SKH-1 hairless mice were divided into three groups: control (N = 7), distilled water- (N = 10), and P. multiflorum extract-treated (PM, N = 10) groups. The PM (10 g) was extracted with 100 mL distilled water, cryo-dried and 9.8 g was obtained. The animals received a topical application of 500 µL distilled water or PM extract (1, 2, 4, 8, and 16%, w/v, dissolved in distilled water) for 30 min after UVB irradiation (wavelength 280-320 nm, 300 mJ/cm²; 3 min) of the dorsal kin for 14 days, and skin immunohistochemistry and Cu,Zn-superoxide dismutase (SOD1) activity were determined. SOD1 immunoreactivity, its protein levels and activities in the skin were significantly reduced by 70% in the distilled water-treated group after UVB irradiation compared to control. However, in the PM extract-treated groups, SOD1 immunoreactivity and its protein and activity levels increased in a dose-dependent manner (1-16%, w/v, PM extract) compared to the distilled water-treated group. SOD1 protein levels and activities in the groups treated with 8 and 16%, w/v, PM extract recovered to 80-90% of the control group levels after UVB. These results suggest that PM extract strongly inhibits the destruction of SOD1 by UV radiation and probably contains anti-skin photoaging agents.

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